Title: The v-sis oncoprotein loses transforming activity when targeted to the early Golgi complex Document date: 1994_12_2
ID: 2otgb2w8_20
Snippet: NIH3T3 cells were transfected as described above with 9 #g of MLV expression constructs plus 1 /~g pZAP helper virus. Cells from individual foci were isolated with cloning rings and transferred to 24-well plates with trypsin, and allowed to grow for 2-3 d, refeeding after 1 d. The cells were trypsinized, transferred to 60-mm plates, then 1 d later split 1:4 to two 10-cm plates, and the following day treated with media with or without 100 #M suram.....
Document: NIH3T3 cells were transfected as described above with 9 #g of MLV expression constructs plus 1 /~g pZAP helper virus. Cells from individual foci were isolated with cloning rings and transferred to 24-well plates with trypsin, and allowed to grow for 2-3 d, refeeding after 1 d. The cells were trypsinized, transferred to 60-mm plates, then 1 d later split 1:4 to two 10-cm plates, and the following day treated with media with or without 100 #M suramin. Cells were examined for morphological changes after 24 h.
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