Title: Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus Document date: 1986_6_1
ID: 4sw25blb_12
Snippet: Protease Treatments. A lysate (0.5 ml) derived from 2 x l06 CHl.l. cells was treated with different amounts (5-50 ~g) of Staphylococcus aureus V8 protease for 15 min at 30"C. Proteolysis was stopped by addition of trichloreacetic acid (TCA) to a final concentration of 10%. The TCA precipitate was washed twice with 10% TCA/50% acetone to remove the detergent and solubilized in 25/~l of sample buffer containing 3% SDS (3). After SDS PAGE, the prote.....
Document: Protease Treatments. A lysate (0.5 ml) derived from 2 x l06 CHl.l. cells was treated with different amounts (5-50 ~g) of Staphylococcus aureus V8 protease for 15 min at 30"C. Proteolysis was stopped by addition of trichloreacetic acid (TCA) to a final concentration of 10%. The TCA precipitate was washed twice with 10% TCA/50% acetone to remove the detergent and solubilized in 25/~l of sample buffer containing 3% SDS (3). After SDS PAGE, the proteins were transferred onto nitrocellulose filters (44) .
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