Selected article for: "horseradish peroxidase and sds polyacrylamide"
Title: Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus
  • Document date: 1986_6_1
    • ID: 4sw25blb_14
    Snippet: lmmunoblotting. Proteins were electrophoretically transferred from SDS polyacrylamide gels onto a nitrocellulose filter (44) . The nitrocellulose filter was washed twice for 20 rain in phosphate-buffered saline (PBS) containing 10% newborn calf serum, then treated for 90 min with cell culture supernatant containing In-1 antibody, washed 4x in PBS/newborn calf serum and once in PBS/newborn calf serum containing 0.05% Triton X-100. Bound antibody w.....
    Document: lmmunoblotting. Proteins were electrophoretically transferred from SDS polyacrylamide gels onto a nitrocellulose filter (44) . The nitrocellulose filter was washed twice for 20 rain in phosphate-buffered saline (PBS) containing 10% newborn calf serum, then treated for 90 min with cell culture supernatant containing In-1 antibody, washed 4x in PBS/newborn calf serum and once in PBS/newborn calf serum containing 0.05% Triton X-100. Bound antibody was detected with rabbit anti-rat lgG coupled to horseradish peroxidase and stained with diaminobenzidine (44) .

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