Author: Sousa, Carole; Golebiewska, Anna; Poovathingal, Suresh K; Kaoma, Tony; Pires-Afonso, Yolanda; Martina, Silvia; Coowar, Djalil; Azuaje, Francisco; Skupin, Alexander; Balling, Rudi; Biber, Knut; Niclou, Simone P; Michelucci, Alessandro
Title: Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures Document date: 2018_9_11
ID: 0662rivp_14
Snippet: In Figure 2 D the authors try to show that there is no monocyte contamination of their sorted microglia population by performing qPCR for Ly6c and Ccr2. The way it is presented does not provide information on the expression levels of these two genes in relation to the positive cell subset (monocytes). A positive control should therefore be included......
Document: In Figure 2 D the authors try to show that there is no monocyte contamination of their sorted microglia population by performing qPCR for Ly6c and Ccr2. The way it is presented does not provide information on the expression levels of these two genes in relation to the positive cell subset (monocytes). A positive control should therefore be included.
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