Selected article for: "control fold change and fold change"

Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice
  • Document date: 2020_1_28
  • ID: ely200x3_42
    Snippet: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL.....
    Document: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL13a as the reference gene. Experimental groups consisted of at least 7 animals. Data were analyzed using the comparative threshold cycle (Ct) method and results were expressed as fold of change from control. Specific oligonucleotide primers for qPCR are as follows: TNF-α, forward, 5'-CTTCTGTCTACTGAACTTCGGG-3', reverse, 5'-CAGGCTTGTCACTCGAATTTTG-3';

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