Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_42
Snippet: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL.....
Document: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL13a as the reference gene. Experimental groups consisted of at least 7 animals. Data were analyzed using the comparative threshold cycle (Ct) method and results were expressed as fold of change from control. Specific oligonucleotide primers for qPCR are as follows: TNF-α, forward, 5'-CTTCTGTCTACTGAACTTCGGG-3', reverse, 5'-CAGGCTTGTCACTCGAATTTTG-3';
Search related documents:
Co phrase search for related documents- primer set and real time system: 1
- primer set and reference gene: 1
- primer set and RT kit: 1, 2, 3, 4, 5, 6, 7
- primer set and show specific primer set: 1
- real time and reference gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50
- real time and rox kit: 1
- real time and RT kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- real time and specific primer set: 1, 2, 3, 4, 5, 6
- real time and threshold cycle: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- real time system and reference gene: 1, 2, 3, 4
- real time system and rox kit: 1
- real time system and RT kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- real time system and specific primer set: 1
- real time system and threshold cycle: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- reference gene and RT kit: 1, 2
- reference gene and threshold cycle: 1, 2, 3, 4, 5, 6
- RT kit and threshold cycle: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- show specific primer set and specific primer set: 1
- specific primer set and threshold cycle: 1
Co phrase search for related documents, hyperlinks ordered by date