Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_42
Snippet: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL.....
Document: Total RNA was extracted from the hippocampus using Aurum Total RNA mini-kit (Bio-Rad, cat#732-6870) and phenol/chloroform mix (Invitrogen, cat#15593-031). cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad) using SensiFAST SYBR NO-ROX kit (Invitrogen, cat#BIO-98020) and specific primer sets (shown below) for TNF-α, IL-1β, IL-6, and RPL13a as the reference gene. Experimental groups consisted of at least 7 animals. Data were analyzed using the comparative threshold cycle (Ct) method and results were expressed as fold of change from control. Specific oligonucleotide primers for qPCR are as follows: TNF-α, forward, 5'-CTTCTGTCTACTGAACTTCGGG-3', reverse, 5'-CAGGCTTGTCACTCGAATTTTG-3';
Search related documents:
Co phrase search for related documents- Applied Biosystems RT kit and Ct method: 1
- Applied Biosystems RT kit and mini kit: 1
- Applied Biosystems RT kit and real time: 1, 2, 3, 4
- Applied Biosystems RT kit and real time system: 1
- Bio Rad real time system and change fold: 1, 2
- Bio Rad real time system and Ct method: 1, 2
- Bio Rad real time system and mini kit: 1, 2
- Bio Rad real time system and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- Bio Rad real time system and real time system: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- Bio Rad real time system and reference gene: 1, 2
- cdna Applied Biosystems RT kit and Ct method: 1
- cdna Applied Biosystems RT kit and real time: 1
- change fold and Ct method: 1, 2, 3, 4, 5, 6
- change fold and experimental group: 1
- change fold and mini kit: 1, 2, 3, 4
- change fold and primer set: 1
- change fold and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- change fold and real time system: 1, 2, 3
- change fold and reference gene: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date