Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation Document date: 2019_8_6
ID: 5xk3z4ck_71
Snippet: U2OS cells or their derivatives were grown on coverslips in 12-or 24-well plates. Cells were transfected and/or treated as indicated and described in the figure legend. After washing the cells with PBS, cells were fixed for 1 min with ice-cold methanol/acetone (1:1). After rehydration, the cells were blocked with 5 % BSA in PBS for 1 h at room temperature......
Document: U2OS cells or their derivatives were grown on coverslips in 12-or 24-well plates. Cells were transfected and/or treated as indicated and described in the figure legend. After washing the cells with PBS, cells were fixed for 1 min with ice-cold methanol/acetone (1:1). After rehydration, the cells were blocked with 5 % BSA in PBS for 1 h at room temperature.
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