Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_63
Snippet: In order to control for potential recognition deficits, an experimental design similar to social memory was used (Prado et al., 2006) . Initially, a 10-min habituation phase was given where the mice explored an empty holed acrylic cylinder in the test cage. Mice then underwent five consecutive 3 min trials with 10 min intertrial intervals. A green rectangular lego block was placed under the cylinder in the first four trials, whereas a red rectang.....
Document: In order to control for potential recognition deficits, an experimental design similar to social memory was used (Prado et al., 2006) . Initially, a 10-min habituation phase was given where the mice explored an empty holed acrylic cylinder in the test cage. Mice then underwent five consecutive 3 min trials with 10 min intertrial intervals. A green rectangular lego block was placed under the cylinder in the first four trials, whereas a red rectangular lego block was used in the last trial. The time spent sniffing the acrylic cylinder (within 5cm; defined by Any-maze software) was measured in each of the five trials. Results are graphed as time (seconds) the mice spent investigating the cylinder during each trial and were analyzed using two-way ANOVA with repeated measures with Bonferroni post hoc analysis.
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