Selected article for: "cell surface and fusion activity"
Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity
  • Document date: 1998_6_15
    • ID: 78fjem8s_50
    Snippet: To explore further the time course of fusion by V55P, we analyzed R18 dequenching using a fluorimetric assay (Kemble et al., 1994; Danieli et al., 1996) . We analyzed the fusion activity of 293T cells expressing different amounts of wt-and V55P-HA. Equal numbers of 293T cells expressed wt-and V55P-HA, but the amount of HA at the cell surface varied with the amount of input DNA (Table II) . As seen in Fig. 8 and Table II , with decreasing amounts.....
    Document: To explore further the time course of fusion by V55P, we analyzed R18 dequenching using a fluorimetric assay (Kemble et al., 1994; Danieli et al., 1996) . We analyzed the fusion activity of 293T cells expressing different amounts of wt-and V55P-HA. Equal numbers of 293T cells expressed wt-and V55P-HA, but the amount of HA at the cell surface varied with the amount of input DNA (Table II) . As seen in Fig. 8 and Table II , with decreasing amounts of wt-HA, the rate of fusion decreased. The rate of fusion for V55P was considerably slower, however, than for all levels of wt-HA tested. When normalized for HA density, the relative initial rate of fusion of V55P was found to be ‫ف‬ 20-40% that of wt-HA (Table II) .

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