Selected article for: "dna sequencing and reading frame"
Author: Firth, Andrew E.; Wills, Norma M.; Gesteland, Raymond F.; Atkins, John F.
Title: Stimulation of stop codon readthrough: frequent presence of an extended 3' RNA structural element
  • Document date: 2011_4_27
    • ID: 2u49b7xo_11
    Snippet: The sequences encompassing the RT site and the predicted 3 0 stem-loop structure for VEEV and SINV were synthesized by GenScript and cloned into the XhoI and BglII sites of pDluc, a derivative of the p2luc vector (61, 62) . The firefly luciferase gene is in the same reading frame relative to the upstream renilla luciferase gene such that RT of the stop codon results in a renilla-firefly luciferase fusion product. Derivative constructs were genera.....
    Document: The sequences encompassing the RT site and the predicted 3 0 stem-loop structure for VEEV and SINV were synthesized by GenScript and cloned into the XhoI and BglII sites of pDluc, a derivative of the p2luc vector (61, 62) . The firefly luciferase gene is in the same reading frame relative to the upstream renilla luciferase gene such that RT of the stop codon results in a renilla-firefly luciferase fusion product. Derivative constructs were generated by PCR using appropriate primers and recloning into pDluc. All plasmids were verified by DNA sequencing.

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