Author: Cobucci-Ponzano, Beatrice; Conte, Fiorella; Benelli, Dario; Londei, Paola; Flagiello, Angela; Monti, Maria; Pucci, Piero; Rossi, Mosè; Moracci, Marco
Title: The gene of an archaeal a-l-fucosidase is expressed by translational frameshifting Document date: 2006_8_18
ID: 69gftii4_22
Snippet: The plasmids expressing the mutant genes fucA1 A , fucA1 sm and fucA1 tm for experiments of translation in vitro were prepared by substituting the KpnI-NcoI wild-type fragment, containing the slippery site, with those isolated from the mutants. To check that the resulting plasmids had the correct sequence, the mutant genes were completely re-sequenced......
Document: The plasmids expressing the mutant genes fucA1 A , fucA1 sm and fucA1 tm for experiments of translation in vitro were prepared by substituting the KpnI-NcoI wild-type fragment, containing the slippery site, with those isolated from the mutants. To check that the resulting plasmids had the correct sequence, the mutant genes were completely re-sequenced.
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