Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice

William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado

A) Experimental approach and timeline for hippocampus gene expression experiments. CNO (1 mg/kg; i.p.); LPS (0.1 mg/kg; i.p.). B-D) qPCR measurements of mRNA expression of TNF-α (B), IL-1β (C), and IL-6 (D) in hippocampus of hM3Dq-positive mice following vehicle or CNO treatment prior to saline or LPS injection. All data are expressed as folds of change from the vehicle/saline-treated group and are represented as mean ± SEM. ns p>0.05, ****p<0.0001. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.27.921809 doi: bioRxiv preprint assess object recognition. J) Time spent investigating an enclosed scent to test olfaction. Data are represented as mean ± SEM. ns p>0.05. CX3CR1CreER(+/-)-hM3Dq(+/-) mice 2 hr after LPS or saline injection. E-G) qPCR measurements of mRNA expression of TNF-α (E), IL-1β (F), and IL-6 (G) in hippocampus of CX3CR1CreER(+/-) (hM3Dq-negative) mice. All data are expressed as folds of change from the vehicle/saline-treated group and are represented as mean ± SEM. ns p>0.05, *p<0.05, *** p<0.001, **** p<0.0001.