Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome Document date: 2013_3_26
ID: 1pbd4maf_12
Snippet: Locked nucleic acid (LNA)/DNA chimeras were ordered from Exiqon, the sequences of which are provided in Supplementary Table S1. Chimeras were added at 10-fold excess after folding. Samples were subsequently incubated at 37 C for 15 min before NMIA treatment (see earlier in the text). To quantify alterations induced by ai-oligonucleotides, raw data were processed as described earlier in the text. Tertiary interactions were introduced manually into.....
Document: Locked nucleic acid (LNA)/DNA chimeras were ordered from Exiqon, the sequences of which are provided in Supplementary Table S1. Chimeras were added at 10-fold excess after folding. Samples were subsequently incubated at 37 C for 15 min before NMIA treatment (see earlier in the text). To quantify alterations induced by ai-oligonucleotides, raw data were processed as described earlier in the text. Tertiary interactions were introduced manually into RNAstructure version 5.3 generated models (22) .
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