Author: William Binning; Aja E. Hogan-Cann; Diana Yae Sakae; Matthew Maksoud; Valeriy Ostapchenko; Mohammed Al-Onaizi; Sara Matovic; Wei-Yang Lu; Marco A. M. Prado; Wataru Inoue; Vania F. Prado
Title: Chronic hM3Dq signaling in microglia ameliorates neuroinflammation in male mice Document date: 2020_1_28
ID: ely200x3_36
Snippet: Microglia were plated on glass coverslips coated with poly-D-lysine (~2×104 cells/glass) and incubated in DMEM/F12 (Multicell, cat#319-075-CL) containing 10% heat-inactivated fetal bovine serum (Gibco, cat#10438026) and 1× penicillin/streptomycin (Gibco, cat#15140-122) for 1 day. After approximately 24 hours, 5 µM 4-OHT was added and incubated for another 2 days......
Document: Microglia were plated on glass coverslips coated with poly-D-lysine (~2×104 cells/glass) and incubated in DMEM/F12 (Multicell, cat#319-075-CL) containing 10% heat-inactivated fetal bovine serum (Gibco, cat#10438026) and 1× penicillin/streptomycin (Gibco, cat#15140-122) for 1 day. After approximately 24 hours, 5 µM 4-OHT was added and incubated for another 2 days.
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