Selected article for: "chain reaction and complementary deoxyribonucleic acid"
Author: Otomaru, Hirono; Kamigaki, Taro; Tamaki, Raita; Okamoto, Michiko; Alday, Portia Parian; Tan, Alvin Gue; Manalo, Joanna Ina; Segubre-Mercado, Edelwisa; Inobaya, Marianette Tawat; Tallo, Veronica; Lupisan, Socorro; Oshitani, Hitoshi
Title: Transmission of Respiratory Syncytial Virus Among Children Under 5 Years in Households of Rural Communities, the Philippines
  • Document date: 2019_3_11
    • ID: 0jfvik7w_8
    Snippet: We extracted viral ribonucleic acid (RNA) using the QIAamp MinElute Virus RNA Spin kit (QIAGEN, Hilden, Germany). Subsequently, the viral RNA was reverse-transcribed to complementary deoxyribonucleic acid using Moloney murine leukemia virus reverse transcriptase and random primer (Invitorogen, Carlsbad, CA). In addition, real-time polymerase chain reaction (PCR) was performed to screen RSV. Finally, using conventional PCR targeting the G gene, 2 .....
    Document: We extracted viral ribonucleic acid (RNA) using the QIAamp MinElute Virus RNA Spin kit (QIAGEN, Hilden, Germany). Subsequently, the viral RNA was reverse-transcribed to complementary deoxyribonucleic acid using Moloney murine leukemia virus reverse transcriptase and random primer (Invitorogen, Carlsbad, CA). In addition, real-time polymerase chain reaction (PCR) was performed to screen RSV. Finally, using conventional PCR targeting the G gene, 2 RSV subgroups (RSV-A or RSV-B) were determined for RSV-positive samples, as previously described [20] .

    Search related documents:
    Co phrase search for related documents
    • chain reaction and real time polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • chain reaction and real time polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • chain reaction and rsv screen: 1
    • chain reaction and RSV subgroup: 1, 2, 3, 4
    • chain reaction and Spin kit: 1, 2, 3
    • complementary deoxyribonucleic acid and real time: 1
    • complementary deoxyribonucleic acid and real time polymerase: 1
    • complementary deoxyribonucleic acid and real time polymerase chain reaction: 1
    • conventional PCR and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • conventional PCR and real time polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • conventional PCR and real time polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • conventional PCR and Spin kit: 1
    • deoxyribonucleic acid and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • deoxyribonucleic acid and real time polymerase: 1, 2, 3, 4, 5, 6, 7
    • deoxyribonucleic acid and real time polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7
    • leukemia virus and real time polymerase: 1, 2, 3
    • leukemia virus and real time polymerase chain reaction: 1, 2, 3
    • PCR real time polymerase chain reaction and RSV subgroup: 1
    • PCR real time polymerase chain reaction and Spin kit: 1