Author: Otomaru, Hirono; Kamigaki, Taro; Tamaki, Raita; Okamoto, Michiko; Alday, Portia Parian; Tan, Alvin Gue; Manalo, Joanna Ina; Segubre-Mercado, Edelwisa; Inobaya, Marianette Tawat; Tallo, Veronica; Lupisan, Socorro; Oshitani, Hitoshi
Title: Transmission of Respiratory Syncytial Virus Among Children Under 5 Years in Households of Rural Communities, the Philippines Document date: 2019_3_11
ID: 0jfvik7w_8
Snippet: We extracted viral ribonucleic acid (RNA) using the QIAamp MinElute Virus RNA Spin kit (QIAGEN, Hilden, Germany). Subsequently, the viral RNA was reverse-transcribed to complementary deoxyribonucleic acid using Moloney murine leukemia virus reverse transcriptase and random primer (Invitorogen, Carlsbad, CA). In addition, real-time polymerase chain reaction (PCR) was performed to screen RSV. Finally, using conventional PCR targeting the G gene, 2 .....
Document: We extracted viral ribonucleic acid (RNA) using the QIAamp MinElute Virus RNA Spin kit (QIAGEN, Hilden, Germany). Subsequently, the viral RNA was reverse-transcribed to complementary deoxyribonucleic acid using Moloney murine leukemia virus reverse transcriptase and random primer (Invitorogen, Carlsbad, CA). In addition, real-time polymerase chain reaction (PCR) was performed to screen RSV. Finally, using conventional PCR targeting the G gene, 2 RSV subgroups (RSV-A or RSV-B) were determined for RSV-positive samples, as previously described [20] .
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