Author: Sun, Shihui; Jiang, Yuting; Wang, Renxi; Liu, Chenfeng; Liu, Xiaoling; Song, Nianping; Guo, Yan; Guo, Renfeng; Du, Lanying; Jiang, Shibo; Li, Yan; Qiu, Zewu; Zhao, Guangyu; Zhou, Yusen
Title: Treatment of Paraquat-Induced Lung Injury With an Anti-C5a Antibody: Potential Clinical Application* Document date: 2018_4_13
ID: 6ztwho9k_13
Snippet: The blocking efficiency of IFX-1 was tested in a CD11b assay (11) . Damage to the lung tissues was evaluated as previously described (28) . The concentrations of C5a, C3a, and C5b-9 in plasma were measured using human enzyme-linked immunosorbent assay (ELISA) kits (BD Biosciences, San Jose, CA). Measurements of inflammatory cytokines in serum were performed using ELISA kits (U-CyTech Biosciences, Utrecht, The Netherlands; Uscn Life Sciences, Hous.....
Document: The blocking efficiency of IFX-1 was tested in a CD11b assay (11) . Damage to the lung tissues was evaluated as previously described (28) . The concentrations of C5a, C3a, and C5b-9 in plasma were measured using human enzyme-linked immunosorbent assay (ELISA) kits (BD Biosciences, San Jose, CA). Measurements of inflammatory cytokines in serum were performed using ELISA kits (U-CyTech Biosciences, Utrecht, The Netherlands; Uscn Life Sciences, Houston, TX; or eBioscience, Austria, respectively). The analysis of C3c deposition and expression of C3a receptor (C3aR), C5a receptor (C5aR), CD68, myeloperoxidase, surfactant protein A (SP-A), and vascular endothelial-cadherin were detected by immunohistochemistry staining. The relative expression of VE-cadherin was analyzed using the 2 -△△CT method (29) . For detailed information, see Supplemental Material (Supplemental Digital Content 1, http://links.lww.com/CCM/D159).
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