ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation

Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard

(E) Visualization of interacting protein networks in the SINTBAD interactome using the STRING database (Version 11.0). Line thickness indicates the strength of data support. SINTBAD (TBKBP1) is highlighted in dark green and written in bold. Proteins that were tested for their interaction with SINTBAD by co-IP (data not shown) are marked in green or red, indicating a confirmed or unconfirmed interaction, respectively. (F) U2OS cells were transfected to express the SINTBAD interactor PTPN23 alone and were left untreated or exposed to heat shock, followed by the analysis of GFP-PTPN23 localization by fluorescence microscopy. In addition, cells cotransfected to express GFP-PTPN23 and FLAG-SINTBAD were treated the same way. A representative experiment is shown. Scale bar, 10 mm; the percentage of cells showing the displayed phenotype is indicated. The right part schematically summarizes the intracellular localization of the proteins. . Characterization and Regulation of SINT-Speckles (A) U2OS cells were transfected to express the SINTBAD interactor KAT2A alone and were left untreated or exposed to heat shock, followed by the analysis of FLAG-KAT2A localization by fluorescence microscopy. In addition, cells