Selected article for: "fuca1 gene and mutant wild type"
Author: Cobucci-Ponzano, Beatrice; Conte, Fiorella; Benelli, Dario; Londei, Paola; Flagiello, Angela; Monti, Maria; Pucci, Piero; Rossi, Mosè; Moracci, Marco
Title: The gene of an archaeal a-l-fucosidase is expressed by translational frameshifting
  • Document date: 2006_8_18
    • ID: 69gftii4_11
    Snippet: The expression in the E.coli strain BL21(RB791) of the wild-type gene fucA1 and of the mutant genes fucA1 A [previously named FrameFuc in (24) ], fucA1 B , fucA1 sm and fucA1 tm as fusions of glutathione S-transferase (GST) and the purification of the recombinant proteins were performed as reported previously (23) . The nomenclature used in this paper for the different a-fucosidase genes is listed in Table 1 ......
    Document: The expression in the E.coli strain BL21(RB791) of the wild-type gene fucA1 and of the mutant genes fucA1 A [previously named FrameFuc in (24) ], fucA1 B , fucA1 sm and fucA1 tm as fusions of glutathione S-transferase (GST) and the purification of the recombinant proteins were performed as reported previously (23) . The nomenclature used in this paper for the different a-fucosidase genes is listed in Table 1 .

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