Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation Document date: 2019_8_6
ID: 5xk3z4ck_65
Snippet: representing the cytoplasmic fractions were collected in fresh tubes and mixed with 5 × SDS sample buffer. The remaining pellets representing the nuclear/insoluble fractions were washed twice in low-salt buffer and then resuspended in 180 µl 1 × SDS sample buffer, boiled and sheared two times for 20 sec with a sonifier. The purity of the cellular fractions was confirmed by Western blotting, detecting Tubulin and histone H3 as markers for the c.....
Document: representing the cytoplasmic fractions were collected in fresh tubes and mixed with 5 × SDS sample buffer. The remaining pellets representing the nuclear/insoluble fractions were washed twice in low-salt buffer and then resuspended in 180 µl 1 × SDS sample buffer, boiled and sheared two times for 20 sec with a sonifier. The purity of the cellular fractions was confirmed by Western blotting, detecting Tubulin and histone H3 as markers for the cytosolic or nuclear/insoluble fraction, respectively.
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