Selected article for: "sample buffer and SDS sample buffer"
Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation
  • Document date: 2019_8_6
    • ID: 5xk3z4ck_65
    Snippet: representing the cytoplasmic fractions were collected in fresh tubes and mixed with 5 × SDS sample buffer. The remaining pellets representing the nuclear/insoluble fractions were washed twice in low-salt buffer and then resuspended in 180 µl 1 × SDS sample buffer, boiled and sheared two times for 20 sec with a sonifier. The purity of the cellular fractions was confirmed by Western blotting, detecting Tubulin and histone H3 as markers for the c.....
    Document: representing the cytoplasmic fractions were collected in fresh tubes and mixed with 5 × SDS sample buffer. The remaining pellets representing the nuclear/insoluble fractions were washed twice in low-salt buffer and then resuspended in 180 µl 1 × SDS sample buffer, boiled and sheared two times for 20 sec with a sonifier. The purity of the cellular fractions was confirmed by Western blotting, detecting Tubulin and histone H3 as markers for the cytosolic or nuclear/insoluble fraction, respectively.

    Search related documents:
    Co phrase search for related documents
    • fresh tube and sample buffer: 1, 2
    • fresh tube and SDS sample buffer: 1
    • fresh tube and western blotting: 1
    • insoluble fraction and nuclear cytosolic insoluble fraction: 1, 2, 3
    • insoluble fraction and nuclear insoluble fraction: 1, 2, 3, 4, 5, 6
    • insoluble fraction and sample buffer: 1
    • insoluble fraction and western blotting: 1
    • low salt and sample buffer: 1, 2
    • low salt and SDS sample buffer: 1
    • low salt and time shear: 1, 2
    • low salt and western blotting: 1
    • low salt buffer and sample buffer: 1, 2
    • low salt buffer and SDS sample buffer: 1
    • low salt buffer and western blotting: 1
    • nuclear insoluble fraction and sample buffer: 1
    • sample buffer and SDS sample buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65
    • sample buffer and twice wash: 1
    • sample buffer and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • SDS sample buffer and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13