Author: Zakeri, Hamideh; Shokohi, Tahereh; Badali, Hamid; Mayahi, Saba; Didehdar, Mojtaba
Title: Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder Document date: 2015_7_27
ID: 0y3pvht4_22
Snippet: Members of the genus Trichophyton are the most common agents of dermatophytosis in humans and other animals. Identification of Trichophyton species is essentially based on macroscopic and microscopic observations of their morphological features; however, the identification is complicated and laborious owing to the morphological similarity, polymorphism and cultural variability of Trichophyton species. Thus, accurate identification is timeconsumin.....
Document: Members of the genus Trichophyton are the most common agents of dermatophytosis in humans and other animals. Identification of Trichophyton species is essentially based on macroscopic and microscopic observations of their morphological features; however, the identification is complicated and laborious owing to the morphological similarity, polymorphism and cultural variability of Trichophyton species. Thus, accurate identification is timeconsuming and requires a significant level of knowledge and technological expertise (4). ITS-based analyses have found that sequence variation is limited to only one or a few SNPs between certain species, such as between T. tonsurans and T. equinum, and between T. rubrum and T. soudanense (7) . Although sequencing of the ITS region of rDNA is currently the gold standard for the identification of Trichophyton species and relatives, the technique is relatively expensive and time-consuming, and is impractical for the analysis of large numbers of isolates for epidemiological studies. To overcome these problems, molecular biology-based techniques have been developed for rapid and accurate species determination.
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