Author: Hölzer, Martin; Schoen, Andreas; Wulle, Julia; Müller, Marcel A.; Drosten, Christian; Marz, Manja; Weber, Friedemann
Title: Virus- and Interferon Alpha-Induced Transcriptomes of Cells from the Microbat Myotis daubentonii Document date: 2019_8_10
ID: 0co6m9af_55
Snippet: Based on our strand-specific RNA-Seq data and with the help of our de novo assembly, we found various IFN genes to be annotated on the wrong strand in the Ensembl annotation of M. lucifugus. Therefore, these genes got initially zero read counts when estimating expression values with featureCounts (v1.5.0) (Liao et al., 2014) . To also take these genes into consideration, we adjusted the Ensembl annotation and changed the strand from '+' to '-' fo.....
Document: Based on our strand-specific RNA-Seq data and with the help of our de novo assembly, we found various IFN genes to be annotated on the wrong strand in the Ensembl annotation of M. lucifugus. Therefore, these genes got initially zero read counts when estimating expression values with featureCounts (v1.5.0) (Liao et al., 2014) . To also take these genes into consideration, we adjusted the Ensembl annotation and changed the strand from '+' to '-' for the following gene IDs: ENSMLUG00000026947
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