Author: Warnes, Sarah L.; Little, Zoë R.; Keevil, C. William
Title: Human Coronavirus 229E Remains Infectious on Common Touch Surface Materials Document date: 2015_11_10
ID: 4d4l6mzl_30
Snippet: Human coronavirus 229E (HuCoV-229E) and a fetal fibroblast cell line, MRC-5, were supplied by Public Health England (PHE), United Kingdom. Cells were maintained in minimal essential medium (MEM) supplemented with GlutaMax-1, nonessential amino acids, and 5% fetal calf serum and incubated at 37°C and 5% CO 2 . Cells were passaged twice a week using trypsin (0.25%)-EDTA and were not used beyond passage 30 (P30) (which occurred before the onset of .....
Document: Human coronavirus 229E (HuCoV-229E) and a fetal fibroblast cell line, MRC-5, were supplied by Public Health England (PHE), United Kingdom. Cells were maintained in minimal essential medium (MEM) supplemented with GlutaMax-1, nonessential amino acids, and 5% fetal calf serum and incubated at 37°C and 5% CO 2 . Cells were passaged twice a week using trypsin (0.25%)-EDTA and were not used beyond passage 30 (P30) (which occurred before the onset of senescence, but susceptibility to infection diminished greatly from P30). Viral stocks were prepared by infecting cells at multiplicity of infection of 0.01 for 4 to 7 days until a significant cytopathic effect (CPE) was observed. Infected cells were subjected to 3 freeze/thaw cycles, and infectedcell lysate was stored at Ϫ80°C.
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