Selected article for: "Extraction kit and RNA dna"
Author: Duffy, Mark A; Chen, Qi; Zhang, Jianqiang; Halbur, Patrick G; Opriessnig, Tanja
Title: Impact of dietary spray-dried bovine plasma addition on pigs infected with porcine epidemic diarrhea virus
  • Document date: 2018_8_29
    • ID: 0oyzg5tq_18
    Snippet: To determine fecal PEDV shedding, rectal swabs were collected from all pigs on dpi 6 and from dpi 1 to 14 using polyester swabs and stored in 5-mL plastic tubes containing 1-mL sterile saline. Total nucleic acids were extracted from fecal swab suspensions using the MagMax Pathogen RNA/DNA Kit and an automated nucleic acid extraction system (Thermo Scientific Kingfisher Flex, Thermo Fisher Scientific, Pittsburgh, PA) according to the instructions .....
    Document: To determine fecal PEDV shedding, rectal swabs were collected from all pigs on dpi 6 and from dpi 1 to 14 using polyester swabs and stored in 5-mL plastic tubes containing 1-mL sterile saline. Total nucleic acids were extracted from fecal swab suspensions using the MagMax Pathogen RNA/DNA Kit and an automated nucleic acid extraction system (Thermo Scientific Kingfisher Flex, Thermo Fisher Scientific, Pittsburgh, PA) according to the instructions of the manufacturer Figure 1 . Experimental design. Blood was collected at arrival and dpi 0, 7, and 14, and rectal swabs were collected 6 d before challenge, on dpi 1 and daily thereafter. Rectal temperatures were taken every other day starting with 1 d before PEDV inoculation.

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