Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_39
Snippet: Cell culture and transfection. HEK293 cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum. The cells (1 Â 10 4 ) were seeded into 96-well plates and incubated overnight. JetSI-ENDO transfection reagents (Polyplus-transfection, Illkirch, France) were used for the cotransfection of plasmids and RNAs according to the manufa.....
Document: Cell culture and transfection. HEK293 cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum. The cells (1 Â 10 4 ) were seeded into 96-well plates and incubated overnight. JetSI-ENDO transfection reagents (Polyplus-transfection, Illkirch, France) were used for the cotransfection of plasmids and RNAs according to the manufacturer's instructions. Around 2 Â 10 5 maDCs and DCreg were seeded into each well of 24-well plates and incubated overnight, and then transfected with RNAs using INTERFERin (Polyplus-transfection) according to the manufacturer's instructions.
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