Selected article for: "beta TCR alpha and TCR expression"

Author: Banu, Nasirah; Chia, Adeline; Ho, Zi Zong; Garcia, Alfonso Tan; Paravasivam, Komathi; Grotenbreg, Gijsbert M.; Bertoletti, Antonio; Gehring, Adam J.
Title: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
  • Document date: 2014_2_25
  • ID: 44w6omdp_6
    Snippet: Optimizing virus-specific TCR expression in primary human T cells. The wild type alpha and beta TCR pair was originally tested for expression by co-transducing two separate vectors carrying the TCR alpha and beta genes ( Fig. 1A; a 1 b) . To begin optimizing TCR expression, we made stepwise advancements in the TCR constructs based on published literature using 3 different virus-specific TCRs. The TCRs were linked together with a 2A autocleavage s.....
    Document: Optimizing virus-specific TCR expression in primary human T cells. The wild type alpha and beta TCR pair was originally tested for expression by co-transducing two separate vectors carrying the TCR alpha and beta genes ( Fig. 1A; a 1 b) . To begin optimizing TCR expression, we made stepwise advancements in the TCR constructs based on published literature using 3 different virus-specific TCRs. The TCRs were linked together with a 2A autocleavage site and flexible spacer 13, 14 to promote equimolar production of the alpha and beta chains and codon optimized to increase translational efficiency 17 . These constructs were then modified with a nonnative cysteine disulfide bond in the constant region to increase pairing and stability of the exogenous TCR 15, 16 .

    Search related documents:
    Co phrase search for related documents
    • beta alpha and different virus: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • beta alpha and disulfide bond: 1, 2, 3, 4
    • beta alpha and equimolar production: 1
    • beta alpha and optimize virus: 1
    • beta alpha and TCR alpha: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • beta alpha and TCR construct: 1
    • beta alpha and TCR expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • beta alpha and TCR expression optimize: 1, 2
    • beta alpha and TCR pair: 1
    • beta alpha and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51
    • beta alpha and wild type alpha: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • beta alpha chain and equimolar production: 1
    • beta alpha chain and TCR alpha: 1, 2
    • beta alpha chain and TCR expression: 1, 2
    • beta alpha chain and wild type: 1
    • beta alpha chain and wild type alpha: 1
    • beta TCR alpha and constant region: 1
    • beta TCR alpha and different virus: 1, 2
    • beta TCR alpha and disulfide bond: 1, 2