Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_4
Snippet: Artificial gene sequences were synthesized and assembled from stepwise PCR using oligonucleotides, cloned and fully sequence verified by GeneArt AG (Regensburg, Germany). The coding sequences of the C3P3-G1 enzymes and all other constructions were optimized for expression in human cells with respect to codon adaptation index using the GeneOptimizer algorithm (5) ......
Document: Artificial gene sequences were synthesized and assembled from stepwise PCR using oligonucleotides, cloned and fully sequence verified by GeneArt AG (Regensburg, Germany). The coding sequences of the C3P3-G1 enzymes and all other constructions were optimized for expression in human cells with respect to codon adaptation index using the GeneOptimizer algorithm (5) .
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