Selected article for: "expression system and Firefly Luciferase expression plasmid"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_74
    Snippet: Expression of Firefly Luciferase by the C3P3-G1 expression system was investigated in other cell lines, including the Chinese hamster CHO-K1, the human cervix carcinoma HeLa, the human liver HepG2, and the human fibroblasts WI-38 cells. The expression levels by the C3P3-G1 system were compared to the standard CMV-driven Firefly Luciferase expression plasmid, assayed as previously described. Striking variability was observed across the cell types,.....
    Document: Expression of Firefly Luciferase by the C3P3-G1 expression system was investigated in other cell lines, including the Chinese hamster CHO-K1, the human cervix carcinoma HeLa, the human liver HepG2, and the human fibroblasts WI-38 cells. The expression levels by the C3P3-G1 system were compared to the standard CMV-driven Firefly Luciferase expression plasmid, assayed as previously described. Striking variability was observed across the cell types, with best performances of the C3P3-G1 system observed with the CHO-K1 and HepG2 cell lines (Supplementary Figure S18 ). The mechanisms responsible for this variability across the different cell types will be investigated in further studies.

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