Title: The v-sis oncoprotein loses transforming activity when targeted to the early Golgi complex Document date: 1994_12_2
ID: 2otgb2w8_10
Snippet: The parental vector contains an XhoI restriction site just upstream of the v-sis coding sequence. For use in transfection and infection of NIH3T3 cells, DNA fragments encoding the above described v-sis-fusion proteins were subcloned as XhoI-ClaI restriction fragments into the murine leukemia virus (MLV) expression vector pMS177, which was derived from the previously described retroviral vector pDD102 (Bold and Donoghue, 1985) ......
Document: The parental vector contains an XhoI restriction site just upstream of the v-sis coding sequence. For use in transfection and infection of NIH3T3 cells, DNA fragments encoding the above described v-sis-fusion proteins were subcloned as XhoI-ClaI restriction fragments into the murine leukemia virus (MLV) expression vector pMS177, which was derived from the previously described retroviral vector pDD102 (Bold and Donoghue, 1985) .
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