Treatment of Paraquat-Induced Lung Injury With an Anti-C5a Antibody: Potential Clinical Application*

Sun, Shihui; Jiang, Yuting; Wang, Renxi; Liu, Chenfeng; Liu, Xiaoling; Song, Nianping; Guo, Yan; Guo, Renfeng; Du, Lanying; Jiang, Shibo; Li, Yan; Qiu, Zewu; Zhao, Guangyu; Zhou, Yusen

All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the Beijing Institute of Microbiology and Epidemiology (IACUC Permit No. 2015-12) . Cynomolgus monkeys were assigned to treatment and sham treatment groups. Both received an intraperitoneal injection of paraquat diluted in 5 mL of saline (40 mg/kg). One hour later, the sham treatment group (n = 5) received an IV injection of phosphate buffered saline (PBS), whereas the treatment group (n = 5) received IFX-1 (5 mg/ kg in PBS). Another group of cynomolgus monkeys (n = 2) received an intraperitoneal injection of saline (5 mL; normal group). Whole blood was collected at 0, 6, and 16 hours after paraquat administration, and serum and plasma samples were obtained and stored at -80°C. All monkeys were anesthetized with pentobarbital sodium at 16 hours, and necropsies were performed. Lung and other tissues were collected for pathologic and immunologic assay.