Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation Document date: 2019_8_6
ID: 5xk3z4ck_69
Snippet: After blocking the membrane, primary antibodies, diluted in 2 % nonfat dry milk or 5 % BSA (Sigma-Aldrich) in TBST, were incubated overnight at 4 °C. Peroxidase-coupled antibodies (Jackson ImmuoResearch) were diluted 1:5000 in 2 % dry milk in TBST and incubated 2 h at room temperature. Immunoreactive bands were detected using the Western Lightning Plus-ECL reagent (Perkin Elmer) and visualized on a ChemiDoc Imaging System (Bio-Rad)......
Document: After blocking the membrane, primary antibodies, diluted in 2 % nonfat dry milk or 5 % BSA (Sigma-Aldrich) in TBST, were incubated overnight at 4 °C. Peroxidase-coupled antibodies (Jackson ImmuoResearch) were diluted 1:5000 in 2 % dry milk in TBST and incubated 2 h at room temperature. Immunoreactive bands were detected using the Western Lightning Plus-ECL reagent (Perkin Elmer) and visualized on a ChemiDoc Imaging System (Bio-Rad).
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