Selected article for: "complete protease inhibitor and lysis buffer"
Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation
  • Document date: 2019_8_6
    • ID: 5xk3z4ck_74
    Snippet: To identify SINTBAD interactors by mass spectrometry, 293T cells were either transfected with the pcDNA3.1-Flag-SINTBAD expression construct or the empty vector. Four 10 cm dishes for each condition were taken for large-scale immunoprecipitation. Washed cells were harvested and lysed in 1 ml low-salt buffer as described above. The received cytosolic fraction was mixed with 1 ml IGEPAL lysis buffer, containing Complete Protease Inhibitor.....
    Document: To identify SINTBAD interactors by mass spectrometry, 293T cells were either transfected with the pcDNA3.1-Flag-SINTBAD expression construct or the empty vector. Four 10 cm dishes for each condition were taken for large-scale immunoprecipitation. Washed cells were harvested and lysed in 1 ml low-salt buffer as described above. The received cytosolic fraction was mixed with 1 ml IGEPAL lysis buffer, containing Complete Protease Inhibitor

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