Selected article for: "expression vector and mass spectrometry"
Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation
  • Document date: 2019_8_6
    • ID: 5xk3z4ck_74
    Snippet: To identify SINTBAD interactors by mass spectrometry, 293T cells were either transfected with the pcDNA3.1-Flag-SINTBAD expression construct or the empty vector. Four 10 cm dishes for each condition were taken for large-scale immunoprecipitation. Washed cells were harvested and lysed in 1 ml low-salt buffer as described above. The received cytosolic fraction was mixed with 1 ml IGEPAL lysis buffer, containing Complete Protease Inhibitor.....
    Document: To identify SINTBAD interactors by mass spectrometry, 293T cells were either transfected with the pcDNA3.1-Flag-SINTBAD expression construct or the empty vector. Four 10 cm dishes for each condition were taken for large-scale immunoprecipitation. Washed cells were harvested and lysed in 1 ml low-salt buffer as described above. The received cytosolic fraction was mixed with 1 ml IGEPAL lysis buffer, containing Complete Protease Inhibitor

    Search related documents:
    Co phrase search for related documents
    • cytosolic fraction and low salt buffer: 1
    • igepal lysis buffer and low salt: 1
    • igepal lysis buffer and low salt buffer: 1
    • igepal lysis buffer and lysis buffer: 1, 2, 3, 4
    • igepal lysis buffer and mass spectrometry: 1
    • igepal lysis buffer and ml igepal lysis buffer: 1, 2
    • large scale and low salt: 1
    • large scale and low salt buffer: 1
    • large scale and lysis buffer: 1, 2, 3
    • large scale and mass spectrometry: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • low salt and lysis buffer: 1, 2, 3, 4, 5
    • low salt and mass spectrometry: 1, 2
    • low salt and ml igepal lysis buffer: 1
    • low salt buffer and lysis buffer: 1, 2, 3, 4, 5
    • low salt buffer and ml igepal lysis buffer: 1
    • lysis buffer and mass spectrometry: 1, 2, 3, 4
    • lysis buffer and ml igepal lysis buffer: 1, 2
    • mass spectrometry and ml igepal lysis buffer: 1
    • mass spectrometry and sintbad expression: 1