Selected article for: "independent sample and standard deviation"
Author: Robinson, Lary A; Smith, Prudence; SenGupta, Dhruba J; Prentice, Jennifer L; Sandin, Ramon L
Title: Molecular analysis of sarcoidosis lymph nodes for microorganisms: a case–control study with clinical correlates
  • Document date: 2013_12_21
    • ID: 3unap1o9_23
    Snippet: The 16S gene fragment was amplified as previously described. 8 The hsp65 gene was amplified using TB11 and TB12 primers, and the RNA polymerase subunit gene (rpoB) was amplified using MF and MR primers. 9 The amplified products were then sequenced using the Big Dye Sequencing kit (Applied Biosystems, Foster City, CA) using the vendor's recommended protocol. The sequences of two strands were assembled into doubled-stranded contig using Sequencher .....
    Document: The 16S gene fragment was amplified as previously described. 8 The hsp65 gene was amplified using TB11 and TB12 primers, and the RNA polymerase subunit gene (rpoB) was amplified using MF and MR primers. 9 The amplified products were then sequenced using the Big Dye Sequencing kit (Applied Biosystems, Foster City, CA) using the vendor's recommended protocol. The sequences of two strands were assembled into doubled-stranded contig using Sequencher software (Gene Codes, Ann Arbor, MI). The final sequences were used to search the National Center for Biotechnology Information (National Institutes of Health) database using BLAST (Basic Local Alignment Search Tool) to identify the amplified DNA. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 The primary variable to be compared between the sarcoidosis and controls patients is the number of patients in each group with bacterial DNA found in lymph nodes. The N-1 Two Proportion test for comparing independent proportions for small sample sizes is used to compare the results between the two groups. 10 All numerical data is expressed as the mean + standard deviation.

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