Author: Lee, Na-Ra; Kwon, Hyun-Mi; Park, Kkothanahreum; Oh, Sangtaek; Jeong, Yong-Joo; Kim, Dong-Eun
Title: Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13 Document date: 2010_7_29
ID: 1k99yv4i_16
Snippet: any helicases that dissociated from the substrates during unwinding (Supplementary Data). The trap oligonucleotides also prevented reannealing of the displaced bottom strand (Supplementary Data). Under the single-turnover reaction condition, the nsP13 helicase unwinded the dsDNA substrates and generated ssDNA products that were resolved by non-denaturing PAGE ( Figure 1B ). The kinetic time-course of ssDNA accumulation was plotted and fitted to a.....
Document: any helicases that dissociated from the substrates during unwinding (Supplementary Data). The trap oligonucleotides also prevented reannealing of the displaced bottom strand (Supplementary Data). Under the single-turnover reaction condition, the nsP13 helicase unwinded the dsDNA substrates and generated ssDNA products that were resolved by non-denaturing PAGE ( Figure 1B ). The kinetic time-course of ssDNA accumulation was plotted and fitted to an exponential function to obtain the reaction amplitudes and unwinding rates of only the helicases that were initially bound to the DNA substrates.
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