Author: Pipirou, Zoi; Powlesland, Alex S; Steffen, Imke; Pöhlmann, Stefan; Taylor, Maureen E; Drickamer, Kurt
Title: Mouse LSECtin as a model for a human Ebola virus receptor Document date: 2011_1_21
ID: 41i20yuy_31
Snippet: Approximately 250 µg of biotin-tagged CRD in 6 mL of eluting buffer was adjusted to 25 mM CaCl 2 and incubated overnight at 4°C with 150 µg of Alexa 488-labeled streptavidin (Invitrogen). The complex was re-applied to a 1-mL column of mannose-Sepharose, which was rinsed with 5 mL of loading buffer and eluted with 0.5-mL aliquots of eluting buffer. Version 4.0 of the glycan array of the Consortium for Functional Glycomics was screened in buffer.....
Document: Approximately 250 µg of biotin-tagged CRD in 6 mL of eluting buffer was adjusted to 25 mM CaCl 2 and incubated overnight at 4°C with 150 µg of Alexa 488-labeled streptavidin (Invitrogen). The complex was re-applied to a 1-mL column of mannose-Sepharose, which was rinsed with 5 mL of loading buffer and eluted with 0.5-mL aliquots of eluting buffer. Version 4.0 of the glycan array of the Consortium for Functional Glycomics was screened in buffer containing 150 mM NaCl, 2 mM CaCl 2 , 2 mM MgCl 2 , 20 mM Tris-Cl pH 7.4, 0.05% Tween 20 and 1% BSA following their standard protocol.
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