Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays Document date: 2017_9_20
ID: 7t1o19kn_37
Snippet: In addition, we compared the S/B (signal to background) ratios between direct fluorescence and tyramide signal amplification (TSA) using a fluorophore-linked or HRP-conjugated anti-p53 antibody, respectively. Using the signal from "non-spots" as background, we found that the S/B ratio of fluorescence detection using an antibody with a directly-conjugated fluorophore, the Dylight649 rabbit anti-mouse IgG, was higher (S/B ratio = 431±38) than the .....
Document: In addition, we compared the S/B (signal to background) ratios between direct fluorescence and tyramide signal amplification (TSA) using a fluorophore-linked or HRP-conjugated anti-p53 antibody, respectively. Using the signal from "non-spots" as background, we found that the S/B ratio of fluorescence detection using an antibody with a directly-conjugated fluorophore, the Dylight649 rabbit anti-mouse IgG, was higher (S/B ratio = 431±38) than the TSA method with the HRP-labeled goat anti-mouse IgG (S/B ratio = 323±18). Thus, directly-conjugated fluorescent secondary antibodies were used for the following assays ( Figure S8 ).
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