Origin, Evolution, and Virulence of Porcine Deltacoronaviruses in the United States

Ma, Yuanmei; Zhang, Yu; Liang, Xueya; Lou, Fangfei; Oglesbee, Michael; Krakowka, Steven; Li, Jianrong

Gross pathological changes in gnotobiotic piglets infected with the PdCV CVM1 or MI strain. Gross findings were similar in all three Gn piglets inoculated with PdCV Ohio CVM1. The perineum, ventral abdomen, and hind legs were coated with yellow, adherent diarrheic feces (see Fig. S2 ). Dilated gas-and fluidfilled small intestines with thin, translucent walls were observed, and both the stomachs and small intestines contained coagulated liquid milk replacement diet (see Fig. S2 ). The cecum, spiral colon, and terminal colon were dilated and filled with yellow intestinal fluids. In addition to the intestinal changes, ascites, hydrothorax, and thymic atrophy were detected in the piglet terminated at 72 h p.i. The cell culture-adapted PdCV MI strain caused gross pathology similar to that of PdCV Ohio CVM1 in Gn piglets (see Fig. S3 in the supplemental material).