Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method Document date: 2018_3_23
ID: 4nphwznx_21
Snippet: The RVFV structural M gene was obtained by applying the PCR amplification method. The amplified fragment length was 3218 bp. As shown in Fig. 1 , the pUC57-Simple-M recombinant plasmid was used as the amplification template, and the results were assessed by carrying out agarose gel electrophoresis. One band was present at approximately 3200 bp, which was consistent with the size (3218 bp) of the expected target fragment. These results indicated t.....
Document: The RVFV structural M gene was obtained by applying the PCR amplification method. The amplified fragment length was 3218 bp. As shown in Fig. 1 , the pUC57-Simple-M recombinant plasmid was used as the amplification template, and the results were assessed by carrying out agarose gel electrophoresis. One band was present at approximately 3200 bp, which was consistent with the size (3218 bp) of the expected target fragment. These results indicated that the correct target gene band was amplified by PCR.
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