Author: Darwish, Ilyse; Miller, Chris; Kain, Kevin C.; Liles, W. Conrad
Title: Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza Document date: 2012_1_13
ID: 1rktb6yq_18
Snippet: Animal use protocols were reviewed and approved by the University Health Network Ontario Cancer Institute Animal Care Committee, and all experiments were conducted in accordance with institutional guidelines in an animal biosafety level 2 facility. Female C57Bl/6 mice, 9-11 weeks old, were obtained from Jackson Laboratories (Bar Harbor, ME, USA) and maintained under pathogen-free conditions with a 12-hour light cycle. On day 0, while under light .....
Document: Animal use protocols were reviewed and approved by the University Health Network Ontario Cancer Institute Animal Care Committee, and all experiments were conducted in accordance with institutional guidelines in an animal biosafety level 2 facility. Female C57Bl/6 mice, 9-11 weeks old, were obtained from Jackson Laboratories (Bar Harbor, ME, USA) and maintained under pathogen-free conditions with a 12-hour light cycle. On day 0, while under light isofluorane anesthesia, experimental mice were infected via nasal instillation with 10 3 plaque forming units (PFU) of influenza A/WSN/33 (H1N1; WSN/33,) (stock kindly provided by Dr. Eleanor Fish, University Health Network/University of Toronto) in 50 μl PBS. Weight was recorded daily for a maximum of twelve days post-infection, and mice were sacrificed when euthanasia criteria was met (greater than 20% weight loss). Lung tissue was harvested for analysis on day 5 post-infection.
Search related documents:
Co phrase search for related documents- experimental mouse and lung tissue: 1, 2
Co phrase search for related documents, hyperlinks ordered by date