Author: Alves, Christian D.B.T.; Budaszewski, Renata F.; Torikachvili, Marcela; Streck, André F.; Weber, Matheus N.; Cibulski, Samuel P.; Ravazzolo, Ana P.; Lunge, Vagner R.; Canal, Cláudio W.
Title: Detection and genetic characterization of Mamastrovirus 5 from Brazilian dogs Document date: 2018_2_2
ID: 29wagjw8_18
Snippet: Four partial genomes were obtained, namely, MAstV5 Sara/13/BRA, MAstV5 237/13/BRA, MAstV5 GRAV/13/BRA and MAstV5 5617/12/BRA, which were 4980, 5011, 5039 and 5012 nt in length, respectively, excluding the poly(A) tail and the 5 untranslated regions (UTRs). The three first partial genomes were collected from different cities of Rio Grande do Sul State (Porto Alegre, Viamão and GravataÃ, respectively), and one was from Londrina city, Paraná Stat.....
Document: Four partial genomes were obtained, namely, MAstV5 Sara/13/BRA, MAstV5 237/13/BRA, MAstV5 GRAV/13/BRA and MAstV5 5617/12/BRA, which were 4980, 5011, 5039 and 5012 nt in length, respectively, excluding the poly(A) tail and the 5 untranslated regions (UTRs). The three first partial genomes were collected from different cities of Rio Grande do Sul State (Porto Alegre, Viamão and GravataÃ, respectively), and one was from Londrina city, Paraná State. All of the four nearly complete genomes contained a typical AstV organization in the three predicted ORFs -ORF1a, ORF1b and ORF2. Further genome sequence comparison revealed that the four partial genomes had greater identities (94-96%) with Chinese strains, considered as the Italy, Hungary and United Kingdom strains identity ranged from 76-82%, 75-95% and 86-96% when compared to the Brazilian strains identified here, respectively (Table 2 ). In addition, the amino acid sequences of the ORF2 of the HUN/2012/126 (GenBank accession number KX599352), GI.E/Dog/ITA/2010/Zoid (GenBank accession number JN193534) strains showed lower identities (76 and 77%, respectively) compared to the Brazilian strains ( Table 2) .
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