Author: Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung
Title: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera Document date: 2016_9_20
ID: 0pe8vgin_36
Snippet: The results showed that 97.4% of the total serum samples yielded negative results in the IFA; therefore, this group was regarded as negative to SFTSV. The cELISA cutoff value was subsequently calculated using the mean inhibition rate of the IFA-negative population ± 3S.D. (cutoff PI = 49.5%). Using this cut-off, 99.8% of the IFA-negative bovine serum samples were also considered to be negative in the cELISA. Although the newly developed cELISA i.....
Document: The results showed that 97.4% of the total serum samples yielded negative results in the IFA; therefore, this group was regarded as negative to SFTSV. The cELISA cutoff value was subsequently calculated using the mean inhibition rate of the IFA-negative population ± 3S.D. (cutoff PI = 49.5%). Using this cut-off, 99.8% of the IFA-negative bovine serum samples were also considered to be negative in the cELISA. Although the newly developed cELISA is highly specific, more field animals infected with SFTSV would need to be measured to improve the sensitivity.
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