Author: Lew, Qiao Jing; Chu, Kai Ling; Lee, Jialing; Koh, Poh Ling; Rajasegaran, Vikneswari; Teo, Jin Yuan; Chao, Sheng-Hao
Title: PCAF interacts with XBP-1S and mediates XBP-1S-dependent transcription Document date: 2010_9_4
ID: 174q2pjw_9
Snippet: 293T cells were transiently co-transfected with indicated expression plasmids and the cell lysates were prepared 2 days post-transfection for co-immunoprecipitation (Co-IP). To get the high levels of ectopic expression, 293T, a highly transfectable derivative of HEK293, was chosen for the Co-IP study. The IP kit was purchased from Roche and Co-IP was performed according to the manufacturers' instructions. The immunoprecipitated complexes were ana.....
Document: 293T cells were transiently co-transfected with indicated expression plasmids and the cell lysates were prepared 2 days post-transfection for co-immunoprecipitation (Co-IP). To get the high levels of ectopic expression, 293T, a highly transfectable derivative of HEK293, was chosen for the Co-IP study. The IP kit was purchased from Roche and Co-IP was performed according to the manufacturers' instructions. The immunoprecipitated complexes were analyzed by western blotting. Western blotting was carried out according to the standard protocols. All the antibodies used in our study were obtained from Santa Cruz Biotechnology, except the anti-HA antibody (Sigma).
Search related documents:
Co phrase search for related documents- cell lysate and expression plasmid: 1
- co Co IP immunoprecipitation and Co IP immunoprecipitation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- co Co IP immunoprecipitation and Co ip study: 1
- co Co IP immunoprecipitation and IP kit: 1
- Co IP immunoprecipitation and IP kit: 1
- Co ip study and expression plasmid: 1
- ectopic expression and high level: 1, 2
- expression plasmid and high level: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date