Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus Document date: 2017_12_2
ID: 4szmu1dh_11
Snippet: The sensitivity of ICA was evaluated by comparing the detection limit using real-time RT-PCR as previously described with some modifications. 13 Briefly, real-time RT-PCR was carried out in a total volume of 20 µl containing viral RNA, PEDV forward (5'-CGC AAAG ACTG AACC CACTAATTT-3') and reverse (5'-TTG CCTC TGTT GTTA CTTGGAGAT-3') primers, probe (6-carboxyfluorescein.....
Document: The sensitivity of ICA was evaluated by comparing the detection limit using real-time RT-PCR as previously described with some modifications. 13 Briefly, real-time RT-PCR was carried out in a total volume of 20 µl containing viral RNA, PEDV forward (5'-CGC AAAG ACTG AACC CACTAATTT-3') and reverse (5'-TTG CCTC TGTT GTTA CTTGGAGAT-3') primers, probe (6-carboxyfluorescein
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