Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Integrity-The mitochondria play a role in energy production, membrane potential regulation, proliferation and cellular metabolism, and modulating cell death pathways in response to microbial infection (81) . Whether mitochondria are affected by HRSV infection has not been characterized previously. Quantitative proteomic analysis coupled to IPA identified changes in mitochondrial proteins in HRSV-infected cells. The localiza- tion and abundance of representative mitochondrial proteins (highlighted during data analysis) were then investigated in separate experiments using indirect immunofluorescence confocal microscopy. It is of interest to note that in this analysis mitochondrial proteins were also detected in nuclear fractions. This could have been an artifact of the purification process, or it could have been due to the tubular structures that contain mitochondria and project into the nucleus (82) . Certainly, the indirect immunofluorescence confocal analysis of Tom20 distribution in HRSV-infected cells reflected the latter hypothesis (supplemental Fig. 1) .