Author: Tuplin, A.; Evans, D. J.; Buckley, A.; Jones, I. M.; Gould, E. A.; Gritsun, T. S.
Title: Replication enhancer elements within the open reading frame of tick-borne encephalitis virus and their evolution within the Flavivirus genus Document date: 2011_5_27
ID: 0aiaklrn_47
Snippet: To exclude the effect of spontaneous mutations in the 5 0 -and 3 0 -UTRs which contain TBFV promoter and enhancer elements (16) that might compensate for the effect of the SL6-mutations, rescued virus was not passaged prior to phenotype evaluation and key regions of the genome (1-940 and 10206-10927) were sequenced following recovery of each SL6-mutated virus. Only the intended substitutions were present, with no reversions or other compensatory .....
Document: To exclude the effect of spontaneous mutations in the 5 0 -and 3 0 -UTRs which contain TBFV promoter and enhancer elements (16) that might compensate for the effect of the SL6-mutations, rescued virus was not passaged prior to phenotype evaluation and key regions of the genome (1-940 and 10206-10927) were sequenced following recovery of each SL6-mutated virus. Only the intended substitutions were present, with no reversions or other compensatory mutations were observed. The effect of each mutation (reduction from large wild-type plaques of the pGGVs virus to medium, small or pin pointed) was scored if the SL6-mutated strain contained >90% of plaques with the altered morphology. The presence of a minor plaque population (between 1 and 10%) was considered as the inevitable result of the variation inherent R 1 4 5 3 3 3 6 2 4 5 2 6 3 2 8 2 2 9 0 2 | | | | | | | | TBEV L40361 ACG CGU CAA UCC AGA GUC CAA AUG CCA AAU GGA CUC GUG UUG AUG CGC TRQSRVQMPNGLVLMR TBEV C10 . in all RNA viruses, the consequence of a high error rate in the virus RdRp (41) .
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