Author: Tromas, Nicolas; Zwart, Mark P.; Forment, Javier; Elena, Santiago F.
Title: Shrinkage of Genome Size in a Plant RNA Virus upon Transfer of an Essential Viral Gene into the Host Genome Document date: 2014_2_20
ID: 5fejitls_29
Snippet: Rapid Occurrence of Large Genomic Mutations in TEV upon Passaging in N. tabacum 35S::NIb Plants To test whether there will indeed be rapid accumulation of virus variants with large deletions as suggested by estimates of W, we performed serial passage experiments. Here, we chose to perform four serial passages, with each passage having 3 weeks of duration, because genome size appears to be under stronger selection in long passages . Five independe.....
Document: Rapid Occurrence of Large Genomic Mutations in TEV upon Passaging in N. tabacum 35S::NIb Plants To test whether there will indeed be rapid accumulation of virus variants with large deletions as suggested by estimates of W, we performed serial passage experiments. Here, we chose to perform four serial passages, with each passage having 3 weeks of duration, because genome size appears to be under stronger selection in long passages . Five independent lineages of TEV were evolved. After four passages, deletions were detected by RT-PCR in two lineages: Lineage 2 and Lineage 3 ( fig. 2A ). These two deletions (nucleotide positions 7621-8484 and 7046-8500) conserved the reading frame and were situated within the NIb cistron (positions 6982-8517 of the wild-type genome).
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