Selected article for: "chain reaction and rt PCR chain reaction"
Author: Jang, Guehwan; Kim, Seong-Hee; Lee, Yoo Jin; Kim, Seungjoon; Lee, Du Sik; Lee, Kyoung-Ki; Lee, Changhee
Title: Isolation and characterization of Korean porcine deltacoronavirus strain KNU16-07
  • Document date: 2018_7_26
    • ID: 5i8q2rdf_3
    Snippet: Swine testicle (ST) cells (ATCC CRL-1746; ATCC, USA) were cultured in alpha minimum essential medium (-MEM; Invitrogen, USA) containing 5% fetal bovine serum (Invitrogen) and antibiotic-antimycotic solutions (100×; Invitrogen). Small intestinal homogenates and stool specimens that tested PDCoV-positive by reverse transcription polymerase chain reaction (RT-PCR) [4] were selected for virus isolation experiments. PDCoV isolation was attempted o.....
    Document: Swine testicle (ST) cells (ATCC CRL-1746; ATCC, USA) were cultured in alpha minimum essential medium (-MEM; Invitrogen, USA) containing 5% fetal bovine serum (Invitrogen) and antibiotic-antimycotic solutions (100×; Invitrogen). Small intestinal homogenates and stool specimens that tested PDCoV-positive by reverse transcription polymerase chain reaction (RT-PCR) [4] were selected for virus isolation experiments. PDCoV isolation was attempted on ST cells as described previously [3] , but with the modification of adding trypsin (USB, USA) to the virus growth medium to obtain a final concentration of 5 to 10 g/mL. The virus supernatants were purified and examined by using a transmission electron microscope (HT7700; Hitachi High Technologies, Japan) as described previously [9] .

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