Selected article for: "acclimation period and control group"

Author: Jang, Guehwan; Kim, Seong-Hee; Lee, Yoo Jin; Kim, Seungjoon; Lee, Du Sik; Lee, Kyoung-Ki; Lee, Changhee
Title: Isolation and characterization of Korean porcine deltacoronavirus strain KNU16-07
  • Document date: 2018_7_26
  • ID: 5i8q2rdf_5
    Snippet: The pig infection experiments described herein were performed at the KNU Animal Facility and followed guidelines established by its Institutional Animal Care and Use Committee (IACUC No. KNU-2016-2032) as described previously [6, 11] . A total of four newborn piglets at 4 days of age were obtained from a commercial pig farm with no known prior PDCoV/PEDV outbreak or PEDV vaccination. All animals were determined to be free of PEDV as well as free .....
    Document: The pig infection experiments described herein were performed at the KNU Animal Facility and followed guidelines established by its Institutional Animal Care and Use Committee (IACUC No. KNU-2016-2032) as described previously [6, 11] . A total of four newborn piglets at 4 days of age were obtained from a commercial pig farm with no known prior PDCoV/PEDV outbreak or PEDV vaccination. All animals were determined to be free of PEDV as well as free of transmissible gastroenteritis virus and rotavirus. Pigs were randomly assigned to two experimental groups housed in two separated rooms: PDCoV-inoculated group (n = 3) in room 1 and sham-inoculated control group (n = 1) in room 2. Following a 1-day acclimation period, piglets in the challenge group were orally administered 1 mL of 10 5 TCID 50 /mL (TCID 50 , 50% tissue culture infective dose) of KNU16-07-P10 virus. The sham-inoculated pig was administered cell culture media as a placebo.

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